Academic Papers

  • Altered sialylation induced by radiation exposure
    D. Iizuka, S. Izumi, F. Suzuki, and K. Kamiya, Analysis of a lecin microarray identifies altered sialylation of mouse serum glycoproteins induced by whole-body radiation exposure, J. of Radiation Research, rry100,
  • Major Depressive Disorder
    H. Yamagata, S. Uchida, K. Matsuo, K. Harada, A. Kobayashi, M. Nakashima, F. Higuchi, T. Watanuki, T. Matsubara, and Y. Watanabe, Altered plasma protein glycosylation in a mouse model of depression and in patients with major depression, J. of Affective Disorders, June 2018, Vol.233, pp-79-85,
  • Exosomes Glycan Profiling Analysis
    A. Shimoda, Y. Tahara, S. Sawada, Y. Sasaki, and K. Akiyoshi, Glycan profiling analysis using evanescent-field fluorescence-assisted lectin array: Importance of sugar recognition for cellular uptake of exosomes from mesenchymal stem cells., BBRC, 2017 Sept. 23, 491(3)pp701-707. doi:10.1016/j.bbrc.2017.07126.
  • Tissue Glycan Mapping
    X. Zou, M. Yoshida, C. Nagai-Okatani, J. Iwaki, B. Tan, K. Hagiwara, T.Sato, Y. Itakura, E.Noro, H. Kaji, M. Toyoda, Y. Zhang, H. Narimatsu, and A. Kuno, A stadardized method for lectin microarray-based tissue glycome mapping, Sci. Rep., 2017 Mar 6;7:43560. doi: 10.1038/srep43560.
  • Colorectal cancer recurrence and ABA
    K. Nakajima, M. Inomata, H. Iha, T. Endoh, N. Shiraishi, K. Kashima, and S. Kitano, Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis, Cancer Med., 2015 (Feb); 4(2): 293-302. doi: 10.1002/cam4.342.
  • Gastric cancer recurrence and BPL
    T. Futsukaichi, T. Etoh, K. Nakajima, T. Daa, H. Shiroshita, N. Shiraishi, S. Kitano, and M. Inomata, Decreased expression of Bauhinia purpurea lectin is a predictor of gastric cancer recurrence, Surg Today, 2015 Oct; 45(10): 1299-306. doi: 10.1007/s00595-015-1127-1.
  • Fucosylation and Intraductal papillary mucinous neoplasms
    K. Watanabe, M. Ohta, K. Yoda, Y. Komori, Y. Iwashita, K. Kashima, and M. Inomata, Fucosylation is associated with the malignant transformation of intraductal papillary mucinous neoplasms: a lectin microarray-based study, Surg Today, 2016 Oct; 46(10): 1217-23. doi: 10.1007/s00595-015-1299-8.
  • Innate immune responses and Glycosylation
    M. Yamamoto-Hino, M. Muraoka, S. Kondo, R. Ueda, H. Okano, and S. Goto, Dynamic regulation of innate immune responses in Drosophia by Senju-mediated glycosylation, Proc. Natl Acad Sci USA, 2015 May 5; 112(18): 5809-14. doi:10.1073/pnas.1424514112.
  • Biobetter Characterization
    M. Roucka, K.Zimmermann, M. Fido, and A. Nechansky, Application of Lectin Array Technology for Biobetter Characterization: Its Correlation with FcyRIII Binding and ADCC, Microarrays 2017, 6(1); doi:10.3390/microarrays6010001.
  • Muchin-Like Glycopeptides
    G. Artigas, H. Hinou, F. G. Martin, H. J. Gabius, and S. Nishimura, Synthetic Mucin-Like Glycopeptides as Versatile Tools to Measure Effects of Glycan Structure/Density/Position on the Interaction with Adhesion/Growth-Regulatory Galectins in Arrays, Chem. An Asian Journal, DOI: 10:1002/asia.201601420.
  • Cancer and O-Linked Glycosylation of Muc1
    A. Matsuda, A. Kuno, T. Nakagawa, Y. Ikehara, T. Irimura, M. Yamamoto, Y. Nakamura, E. Miyoshi, S. Nakamori, H. Nakanishi, C. Viwatthanasittiphong, P. Srivatanakul, M. Miwa, J. Shoda, and H. Narimatsu, Lectin Microarray-Based Sero-Biomarker Verfication Targeting Aberrant O-Linked Glycosylation on Mucin 1, Anal Chem, 2015 Jul 21; 87(14):7274-81. doi: 10.1021/acs.analchem.5b01329. Epub 2015 Jul 7.
  • Lymph node metastasis of advanced gastric cacer
    K. Yamashita, A. Kuno, A. Matsuda, Y. Ikehara, N. Katada, J. Hirabayashi, H. Narimatsu, M. Watanabe, Lectin Microarray technology identifies specific lectins related to lymph node metastasis of advanced gastric cancer, Gastric Cancer, 2015 Apr.4, PMID: 25840959
  • Cancer Diagnosis
    H. Narimatsu, Synthesiology – English edition Vol.5, No.3, p.201 (Dec. 2012)
  • Glycan Profiling of Choriocarcinoma
    Y. Kobayashi, K. Masuda, K. Banno, N. Kobayashi, K. Umene, Y. Nogami, K. Tsuji, A. Ueki, H. nomura, K. Sato, E. Tominaga, T. Shimizu, H. Saya, and D. Aoki, Glycan profiling of gestational choriocarcinoma using a lectin microarray.
  • Novel Glycan Marker for Diabetic Nephropathy
    K. Inoue, J. Wada, J. Eguchi, A. Nakatsuka, S. Teshigawara, K. Murakami, D. Ogawa, T. Terami, A. Katayama, A. Tone, I. Iseda, K. Hida, M. Yamada, T. Ogawa, and H. Makino, Urinary Fetuin-A is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identifies by Lectin Microarray.
  • Lectin Microarrays and glycodiagnostic agent
    A. Kuno, T. Sato, H. Shimazaki, S. Unno, K. Saitou, K. Kiyohara, M. Sogabe, C. Tsuruno, Y. Takehama, Y. Ikehara, and H. Narimatsu, Reconstruction of a robust glycodiagnostic agent supported by multiple lectin-assisted glycan profiling, Proteomics Clin Appl., 2013 May3, doi: 10.1002/prca.201300010.
  • Cancer Marker for Cholangiocarcinoma
    A. Matsuda, A. Kuno, H. Matsuzaki, T. Kawamoto, T. Shikanai, Y. Nakanuma, M. Yamamoto, N. Ohkohchi, Y. Ikehara, J. Shoda, J. Hirabayashi, and H. Narimatsu, Glycoproteomics-based cancer marker discovery adopting dual enrichment with WFA for high specific glyco-diagnosis of cholangiocarcinoma, J. of Proteomics 85 (2013) p.1-11.
  • Cancer Marker for Hepatocellular Carcinoma
    H. Kaji, M. Ocho, A. Togayachi, A. Kuno, M. Sogabe, T. Ohkura, H. Nozaki, T. Angata, Y. Chiba, H. Ozaki, J, Hirabayashi, Y. Tanaka, M. Mizokami, Y. Ikehara, and H. Narimatsu, J. of Proteome Res., 2013, 12 p.2630-2640.
  • Glycan Profiling of Retinal Degradation
    Lectin Microarray Profiling and Relative Quantification of Glycome Associated With Proteins of Neonatal wt and rd1 Mice Retinae, S. Ahuja, Invest Ophathalmol Vis Sci. 2013 May 7; 54(5): 3272-80. doi: 10.1167/iovs.12-11363.
  • Xenotransplant from pig to human
    A lectin array analysis for wild-type and α-Gal-knockout pig islets versus healthy human islets, S. Miyagawa, A. Maeda, S. Takeishi, T. Ueno, N. Usui, S. Matsumoto, T. Okitsu, M. Goto, and H. nagashima, Surg Today, 2013 Apr.3.
  • Adult T-cell Leukemia
    Glycan Profiling of Adult T-Cell Leukemia (ATL) Cells with the High Resolution Lectin Microarrays, H. Iha and M. Yamada, “T-Cell Leukemia – Characteristics, Treatment and Prevention”, book edited by Mariko Tomita, ISBN 978-953-51-0996-9, Published: February 20, 2013 under CC BY 3.0 license, DOI: 10.5772/55386.
  • Lectin Microarrays
    Lectin Microarrays: concept, principle and applications, J. Hirabayashi, M. Yamada, A. Kuno and H. Tateno, Chemical Society Reviews, 2013, doi:10.1039/C3CS35419A.
  • Liver Fibrosis and Glyco-biomarker
    A serum “sweet-doughnut” protein facilitaes fibrosis evaluation and therapy assessment in patients with viral hepatitis, A. Kuno, Y. Ikehara, Y. Tanaka, K. Ito, A Matsuda, S. Sekiya, S. Hige, M. Sakamoto, M. Kaga, M. Mizokami and H. Narimatsu, Scientific Reports 3, Article number: 1065, doi:10.1038/srep01065.
  • Dynamic glycome shift on iPS
    Structural and quantitative evidence for dynamic glycome shift on production of induced pluripotent stem cells, K. Hasehira, H. Tateno, Y. Onuma, Y. Ito, M. Asashima, and J. Hirabayashi, Mol Cell Proteomics., 2012 Dec;11(12):1913-23. doi: 10.1074/mcp.M112.020586.
  • Endometrial cancers
    Glycan profiling of endometrial cancers using lectin microarray, Y. Nishijima, M. Toyoda, M. Yamazaki-Inoue, T. Sugiyama, M. Miyazawa, T. Muramatsu, K. Nakamura, H. Narimatsu, A. Umezawa, and M. Mikami, Genes Cells, 2012 Oct. 17(10):826-36. doi: 10.1111/gtc. 12003.
  • Barrett’s esophagus
    Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett’s esophagus, Bird-Lieberman EL, Neves AA, Lao-Sirieix P, O’Donovan M, Novelli M, Lovat LB, Eng WS, Mahal LK, Brindle KM, Fitzgerald RC., Nature Med. 2012 Jan 15. doi: 10.1038/nm.2616.
  • Application to iPS
    Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis, YC. Wang, M. Nakagawa, I Garitaonandia, I. Slavin, G. Altun, RM Lacharite, KL Nozor, HT Tran, CL Lynch, TR Leonardo, Y. Lue, SE Peterson, LC Laurent, S. Yamanaka, and JF Loring, Cell Res. 2011 Nov;21(11):1551-63. doi: 10.1038/cr.2011.148. Epub 2011 Sep 6.
  • A triplex lectin-santibody sandwich immunoassay for liver fibrosis:
    LecT-Hepa: A triplex lectin-antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside clinical chemistry analyzer and an automated pretreatment machine, A. Kuno, Y. Ikehara, Y. Tanaka, K.Saito, K. Ito, C. Tsuruno, S. Nagai, Y. Takahama, M. Mizokami, J. Hirabayashi, and H. Narimatsu, Clinica Chimica Acta 412 (2011) 1767-1772.
  • Lectin Microarray Profiling of PSGL-1/mIgG:
    Pichia pastoris-produced mucin-type fusion proteins with multivalent O-glycan subsitution as targeting molecules for mannose-specific receptors of the immune system, A. Gustafsson, M. Sjoblom, L. Strindelius, T. Johansson, T. Fleckenstein, N. Chatzissavidou, L. Lindberg, J. Angstrom, U. Rova, and J. Holgersson, Glycobiology, 2011 Aug. 21(8), 1071-1086.
  • Glycan Profiling of Intestinal Bacteria:
    Lectin microarray reveals binding profiles of Lactobacillus casei strains in a comprehensive analysis of bacterial cell wall polysaccharides, E. Yasuda, H. Tateno, J. Hirabayashi, I. Iino, and T.Sako, Appl. Eviron Microbiol., 2011 Jul. 77(13), 4539-4546.
  • Lectin Microarray Profiling of Breast Cancer:
    Lectin Microarray Profiling of Metastatic Breast Cancers, S. A. Fry, B. Afrough, H. J. Lomax-Browne, J. F. Timms, L. S. Velentzis, A. J.C. Leathem, Glycobiology (2011) doi: 10.1093/glycob/cwr045, First published online: April 19, 2011.
  • A possible biomarker for iNPH
    A unique N-glycan on human transferrin in CSF: a possible biomarker for iNPH, S. Futakawa, K. Nara, M. Miyajima, A. Kuno, H. Ito, H. Kaji, K. Shirotani, T. Honda, Y. Tohyama, K. Hoshi, Y. Hanzawa, S. Kitazume, R. Imamaki, K. Furukawa, K. Tasaki, H. Arai, T. Yuasa, M. Abe, H. Arai, H. Narimatsu, and Y. Hashimoto, Neurobiol Aging. 2011 Apr 1. [Epub ahead of print]
  • Lectin Microarray analysis of MSC, ES, and iPS:
    Lectin microarray analysis of pluripotent and multipotent stem cells, M. Toyoda, M. Yamazaki-Inoue, Y. Itakura, A. Kuno, To. Ogawa, M. Yamada, H. Akutsu, Y. takahashi, S. Kanzai, H. Narimatsu, J. Hirabayashi, and A. Umezawa, Genes to Cells, Vol.16, Issue 1, 1-11, January (2011), DOI: 10.1111/j.1365-2443.2010.01459.x
  • Stem Cell Characterization by the Cell Surface Glycome:
    Potential Linkages Between the Inner and Outer Cellular States of Human Induced Pluripotent Stem Cells, S. Saito, Y. Onuma, Y. Ito, H. Tateno, M. Toyoda, H. Akutsu, K. Nishino, E. Chikazawa, Y. Fukawatase, Y. Miyagawa, H. Okita, N. Kiyokawa, Y. Shimma, A. Umezawa, J. Hirabayashi, K. Horimoto, and M. Asashima, ISB2010, Suzhou, China, September 9-11, 2010, pp.381-388.
  • New GlycoBiomarker for Cholangiocarcinoma:
    Wisteria floribunda Agglutinin-Positive Mucin 1 is a Sensitive Biliary Marker for Human Cholangiocarcinoma, A. Matsuda, A. Kuno, H. Matsuzaki, T. Irimura, Y. Ikehara, Y. Zen, Y. Nakamura, M. Yamamoto, N. Ohkuhchi, J. Shoda, J. Hirabayasi, and H. Narimatsu, Hepatology. 2010 Jul;52(1):174-82.
  • Xenotransplantatio of GalT knockout pig:
    S. Miyagawa, S. Takeishi, A. Yamamoto, K. Ikeda, H. Matsunari, M. Yamada, M. Okabe, E. Miyoshi, M. Fukuzawa, and H. nagashima, Xenotransplantation, 2010 Jan. Vol 17(1), pp.61-70.
  • Relationship between activities of IL23R-Fc protein and its glycan structure:
    Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure, K. F. Suen, M. S. Turner, F. Gao, B. Liu, A. Althage, A. Slavin, W. Ou, E. Zuo, M. Eckart, T. Ogawa, M. Yamada, T. Tuntland, J. L. harris, and J. W. Trauger, Protein Expr. Purif. (2010), doi:10.1016/j.pep.2009.12.015.
  • A Strategy for Discovery of Cancer Glyco-biomarkers:
    A Strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics, H. Narimatsu, H. Sawaki, A. Kuno, H. Kaji, H. Ito, and Y.Ikehara, FEBS Journal (2009), doi:10.1111/j.1742-4658.2009.07430.x
  • GPIase Activity of tACE:
    Testicular Angiotensin-Converting Enzyme with Different Glycan Modification: Characterization on Glycosylphosphatidylinositol-Anchored Protein Releasing and Dipeptidase Activities, G. Kondoh, Ho. Watanabe, Y. tashima, Y. Maeda, and T. Kinoshita, J. Biochem. 2009 145(1)115-121, doi:10.1093/jb/mvn148.
  • A Potential Marker for Hepatic progenitor cells:
    High levels of E4-PHA-reactive oligosaccharides: potential as marker for cells with characteristics of hepatic progenitor cells, N. Sasaki, K. Moriwaki, N.Uozomi, K. Noda, N. Taniguchi, A. Kameyama, H. Narimatsu, S. Takeishi, M. Yamada, N. Koyama, and E. Miyoshi, Glyconj J., DOI 10.1007/s10719-009-9240-2, May (2009).
  • Strategy for Glycoproteomics:
    Strategy for Glycoproteomics: Identification of Glyco-Alteration Using Multiple Glycan Profiling Tools (dagger), H. Ito, A. Kuno, H. Sawaki, M. Sogabe, H. Ozaki, Y. Tanaka, M. Mizokami, JI. Shoda, T. Angata, T. Sato, J. Hirabayashi, Y. Ikehara, and H. Narimatsu., J Proteome Res., Mar 6;8(3), pp.1358-1367 (2009).
  • Advanced Antibody-overlay Lectin Microarray Method:
    Focused differential glycan analysis with the platform antibody-assisted lectin profiling (ALP) for glycan-related biomarker verification. A. Kuno, Y. Kato, A. Matsuda, MK. Kaneko, H. Ito, K. Amano, Y. Chiba, H. Narimatsu, and J. Hirabayashi, Mol Cell Proteomics, Jan;8(1), p.99., 2009.
  • Optimization of High-sensitive Detection:
    Optimization of evanescent-field fluorescense-assisted lectin microarray for high-sensitive detection of monovalent oligozaccharides and glycoproteins, Noboru Uchiyama, Atsushi Kuno, Hiroaki Tateno, Yoshiko Kubo, Mamoru Mizuno, Midori Noguchi, and Jun Hirabayashi, Proteomics Vol.8, p.3042 (2008).
  • Development of Data-mining System:
    Development of a data-mining system for differential profiling of cell glycoproteins based on lectin microarray, Atsushi Kuno, Yoko Itakura, Masashi Toyoda, Yoriko Takahashi, Masao Yamada, Akihiro Umezawa, and Jun Hirabayashi, Journal of Proteomics & Bioinformatics(JPB), Vol.1, p.68 (2008.5). Download: 834KB
  • A Review Paper:
    Concept, Strategy and Realization of Lectin-based Glycan Profiling, J. Hirabayashi, J. Biochem. 144, pp.139-147 (2008).
  • Glycan Profiling of Paraffin-embedded Tissue Arrays:
    Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections, Atsushi Matsuda, Atsushi Kuno, Hiroyasu Ishida, Toru Kawamoto, Jun-ichi Shoda and Jun Hirabayashi, Biochemical and Biophysical Research Communications,370, p.259 (2008).
  • The Era of Glycan Profiling has come:
    Glycomics’s Infinite Potential and Applications to Healthcare, M. Yamada, GOR, Vol.9, No.1, p.16 (2007). Download: 2.3MB
  • Profiling of Living Cell Surface Glycome:
    A novel strategy for mammalian cell surface glycome profiling using lectin microarray, H. Tateno, T. Sato, H. Narimatsu, and J. Hirabayashi, Glycobiology, Vol.17, No.10, p.1138 (2007).
  • Antibody-overlay Lectin Microarray Method:
    Inhibition of tumor cell-induced platelet aggregation using a novel anti-podaplanin antibody reacting with its platelet-aggregation-stimulating domain, Y. Kato, M. K. Kaneko, A. Kuno, N. Uchiyama, K. Amano, Y. Chiba, Y. Hasegawa, J. Hirabayashi, H. Narimatsu, K. Mishima, and M. Osawa, doi:10.1016/j.bbrc.2006.08.171.
  • Application to Crude Samples:
    Application of Lectin Microarray to Crude Samples: Differential Glycan Profiling of Lec MutantsF Youji Ebe, Atsushi Kuno, Noboru Uchiyama, Shiori Koseki-Kuno, Masao, Takashi Sato, Hisashi Narimatsu and Jun Hirabayashi, J Biochem, 139(3), p.323 (2006).
  • Frontal Affinity Chromatography:
    High-throughput analysis of lectin-oligosaccharide interactions by automated frontal affinity chromatography, Methods, S. Nakamura-Tsuruta, N. Uchiyama, and J. Hirabayashi, Enzymol., Vol.415, p.311 (2006).
  • Principle of Glycan Profiling Technology and the Performance:
    Evanescent-field fluorescence-assisted lectin microarray: a new strategy for glycan profiling: Atsushi Kuno, Noboru Uchiyama, Shiori Koseki-Kuno1, Youji Ebe, Seigo Takashima, Masao Yamada & Jun Hirabayashi, Nature Methods Vol.2, No.11, p.851 (2005).