A strategy for discovery of new glycobiomarkers is well summarized in a paper written by Dr. H. Narimatsu et al. The following is an explanation of the simplified pipeline for the new glycobiomarker discovery. In short, the merits of using lectin microarrays in a glycobiomarker discovery are in the high sensitityty of GlycoStation and its high throughput operation. For instance, GlycoStation is able to detect faint changes in glycan structures from a few mL of serum samples with using an antibody overlay lectin microarray method, and therefore, this is quite helpful in screening glycobiomarkers from a few dozen of cadidates using precious clinical samples.
Pipeline of GlycoBiomarker Discovery & Screening
Select principal lectins which are sensitive to change in glycan epitopes, using well characterized samples and a conventional lectin microarray analysis method.
Select carrier proteins with referring to existing databases
and/or using IGOT LC-MS/MS.
Enrich glycobiomarker candidates by suing lectins selected in the above and antibodies for the above carrier proteins.
Narrow down candidates into a few with high throughput screening by the antibody overlay lectin microarray method
Identify structures for both protein and glycan by LC-MS/MS
Develop a high performance probe for the glycobiomarker
Develop an assey kit using the new probe or usable lectin
IGOT LC-MS is a way of identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site-specific stable isotope tagging
What we can offer?
GlycoStation™ is such a powerful glycan profiling system consisting of Glycostation™ Reader 1200 (Evanescent-filed fluorescence excitation profiler), LecChip™(Lectin Microarray), and GlycoStation™ Tools Pro (Analysis Software). You can see the detaled information from our main web-site about the products. We can also offer glycan analysis service for your convenience.