Unprecedented Powerful Characterization Method for Stem Cells (ES, iPS, MSC)
Are you satisfied with existing cell surface markers? Identification and characterization of stem cells is a most challenging problem to standardize Stem cell therapy (ES, iPS, MSC etc.). Especially, that of iPS affects an air of seriousness that we need to understand the true meaning of cell reprograming and to establish how to differentiate stem cells into desired states avoiding differentiation into carcinoma.
GlycoTechnica recommends to use Cell Surface Glycome Profiling as an unprecededted powerful characterization method for Stem cells. You may be not so familier with cell surface glycome, but a group of National Center for Child Health Development (NCCHD) and National Institute of Advanced Industrial Science and Technology (AIST) has already reported the potential in ISSCR 2009, and the results were inherited by NEDO iPS Project.(in Japanese), bringing in a lot of new insights furthermore.
A recent paper witten by Dr. Saito and Prof. Asashima et al. must be the first one which uncovers the relationships between gene expression patterns and cell surface glycome profilings in iPS, and another paper written by Dr. Toyoda and Dr. Umezawa et al. explains the performance of LecChip in characterizing pluripotent and multipotent stem cells.
Checking the phenotypes in terms of cell surface glycome is easier and simpler than investigating transcriptome and epigenetics, bacause glycan is the face of cell which intervenes cell-to-cell communication.
The profiling of cell surface glycome is so easy. What you have to do is only Cy3 fluorescence labeling onto cell lysate (prefer cell membrane fraction to whole cell lysate to improve accuracy). Glycostation is a trademark of GlycoTechnica’s glycan profiling system using an evanescent-field fluorescence excitation profiler (GlycoStation™ Reader 1200) and lectin microarrays (LecChip™).
Stem Cell Analysis flow: from Cy3 labeling to Primary Component Analysis
Cy3 labeling and incubation on Lectin Microarray (LecChip™)
Scan fluorescence image with Evanescent-field excitation profiler (GlycoStation™ Reader 1200)
Primary component analysis can discriminate cell states clearly.
Remarks: mES=mouse embryonal stem cells, F9=mouse embryonal carcinoma cells, RA=retinoic acid.
What we can offer?
GlycoStation™ is such a powerful glycan profiling system consisting of Glycostation™ Reader 1200 (Evanescent-filed fluorescence excitation profiler), LecChip™(Lectin Microarray), and GlycoStation™ Tools Pro (Analysis Software). You can see the detaled information from our main web-site about the products. We can also offer glycan analysis service for your convenience.