Glycan Profiling Analysis Tools using Lectin Microarrays
Glycosylation is a common posttranslational modification of proteins where glycan chains are added to a protein molecule. Most of proteins are glycosylated resulting in changes in the protein such as folding, targeting, ligand recognition, binding, stability, immunogenicity, biological activity, etc.
Today, glycans play a significant biological role in four major medical areas; cancer, immunological disease, infectious disease and regenerative medicine. Glycoproteins show promise in the area of cancer biomarkers. Approximately 50% of current cancer markers widely used in clinical cancer diagnosis, are, in fact, various types of Glyco-markers. It is common knowledge in the infectious disease community that most communicable viruses target receptor glycoproteins formed on cell membranes. This fact provides drug developers with opportunities to develop vaccines directed at these target glycoproteins. These are only a few of the many examples that illustrate the need for a quick, simple and highly sensitive method for glycan profiling analysis which can then be used by the biopharmaceutical industry to accelerate the development of advanced biomarkers, thereapeutic proteins, stem cell (iPS, ES, and MSC) characterization, and also probiotics methodologies; all leading to improved diagnostics, therapeutic treatments, and health enhancement.GlycoStation™ is a Gold Standard in Glycan Profiling
GlycoStation™ Evanescent-field Fluorescence Excitation Glycan Profiler
GlycoStation™ Reader 1200 is an ultra-high speed and highly sensitive optical microarray scanner utilizing the principle of evanescent-field fluorescence excitation to scan an entire microarray slide in only two minutes.
A successor model named “GlycoStation™ Reader 2200” was released in the end of June, 2016.
The scan time is to be greatly improved from 1min40sec to only 10sec at 1sec exposure time condition (Sept., 2017)
GlycoLite™ 2200, Evanescent-field Fluorescence Excitation Glycan Profiler
GlycoLite™ 2200 is a low cost version of GlycoStation™ Reader 1200 based on the same principle of evanescent-field fluorescence excitation to scan LecChip Ver.1.0.
This product was released in Apr, 2017. The appearance and specifications are subject to change.
Comparative chart between GlycoStation™ Reader 2200 and GlycoLite™ 2200 is shown below.
|GlycoStation™ Reader 2200||GlycoLite™ 2200|
|Scanning method||Step & Repeat, sCMOS||Step & Repeat, sCMOS|
|Exposure time||133msec – 10sec||133msec – 10sec|
|Excitation method||Evanescent-field Excitation||Evanescent-field Excitation|
|Liquid phase detection||possible||possible|
|Scanning area||13x65mm Standard, 24x65mm at the Maximum||13x65mm|
|Resolution||6.5 µm||6.5 µm|
|Scanning time||10sec at the minimum Exposure time, 80sec at the maximum exposure time||2min35sec at 1sec Exposure time, 9min20sec at the maximum exposure time|
|Light source||Metal halide lamp||LED|
|Fluorescence wavelength||Cy3, Cy5, FITC (up to 6)||Cy3 monochromatic|
|Analysis software (Option)||GlycoStation ToolsPro||GlycoStation ToolsPro|
|Data storage||HDD, Exportable through LAN or USB HDD||HDD, Exportable through LAN or USB Memory|