Glycan Profiling Analysis Tools using Lectin Microarrays
GlycoStation® is a common posttranslational modification of proteins where glycan chains are added to a protein molecule. Most of proteins are glycosylated resulting in changes in the protein such as folding, targeting, ligand recognition, binding, stability, immunogenicity, biological activity, etc.
Today, glycans play a significant biological role in four major medical areas; cancer, immunological disease, infectious disease and regenerative medicine. Glycoproteins show promise in the area of cancer biomarkers. Approximately 50% of current cancer markers widely used in clinical cancer diagnosis, are, in fact, various types of Glyco-markers. It is common knowledge in the infectious disease community that most communicable viruses target receptor glycoproteins formed on cell membranes. This fact provides drug developers with opportunities to develop vaccines directed at these target glycoproteins. These are only a few of the many examples that illustrate the need for a quick, simple and highly sensitive method for glycan profiling analysis which can then be used by the biopharmaceutical industry to accelerate the development of advanced biomarkers, thereapeutic proteins, stem cell (iPS, ES, and MSC) characterization, and also probiotics methodologies; all leading to improved diagnostics, therapeutic treatments, and health enhancement. GlycoStation® is a Gold Standard in Glycan Profiling
GlycoStation® Evanescent-field Fluorescence Excitation Glycan Profiler
GlycoStation® Reader 1200 is an ultra-high speed and highly sensitive optical microarray scanner utilizing the principle of evanescent-field fluorescence excitation to scan an entire microarray slide in only two minutes.
A successor model named “GlycoStation® Reader 2200” was released in the end of June, 2016.
The scan time is to be greatly improved from 1min40sec to only 10sec at 1sec exposure time condition (Sept., 2017)
The new GlycoStation® Reader 2300, which integrates the feature of the GlycoStation® Reader 2200 system, was released in June, 2021.
GlycoStation® Reader 2300 was developed with the support of the Project for Utilizing Glycans in the Development of Innovative Drug Discovery Technologies (grant number JP19ae0101024) from the Japan Agency for Medical Research and Development (AMED).
GlycoSuperLite® 2200, Evanescent-field Fluorescence Excitation Glycan Profiler
GlycoSuperLite® 2200 was released in January, 2022, which is the low-end model of the microarray scanner based on the same principle of GlycoStation® Reader.
This scanner achieved high-speed scanning of LecChip® in just 10 seconds per one glass slide, and became increasingly smaller and lighter than the last model, GlycoLite® 2200.
Comparative chart between GlycoStation® Reader 2300 and GlycoSuperLite® 2200 is shown below.
GlycoStation®ToolsPro Ver.3.0, exclusive software for data analyses
GlycoStation® Tools Pro Ver.3.0 consists of GlycoStation® SignalCapture 3.0 and GlycoStation®Tools Pro 3.0. SignalCapture 3.0 is software to digitalize TIFF files which are scanned images of LecChip® with GlycoStation® Reader. In the SignalCapture 3.0, you can digitalize images with easy-to-use automatic grids generation and adjusting function using Local Background or Global Background. GlycoStation®Tools Pro 3.0 is software to analyze glycan profiling digitized from LecChip® images. In the GlycoStation® Tools Pro 3.0, you can expand the dynamic range of the data※1, draw a graph the lectin signals, display the binding curves, and adjust the data by using several normalization methods※2.
※1Kuno et al., 2008, Journal of Proteomics & Bioinformatics, 1: 68-72
※2 Tateno et al., 2010, Methods in Enzymology, 478: 181-195