Glycan Profiling Analysis Tools using Lectin Microarrays

Glycosylation is a common posttranslational modification of proteins where glycan chains are added to a protein molecule. Most of proteins are glycosylated resulting in changes in the protein such as folding, targeting, ligand recognition, binding, stability, immunogenicity, biological activity, etc.

Today, glycans play a significant biological role in four major medical areas; cancer, immunological disease, infectious disease and regenerative medicine. Glycoproteins show promise in the area of cancer biomarkers. Approximately 50% of current cancer markers widely used in clinical cancer diagnosis, are, in fact, various types of Glyco-markers. It is common knowledge in the infectious disease community that most communicable viruses target receptor glycoproteins formed on cell membranes. This fact provides drug developers with opportunities to develop vaccines directed at these target glycoproteins. These are only a few of the many examples that illustrate the need for a quick, simple and highly sensitive method for glycan profiling analysis which can then be used by the biopharmaceutical industry to accelerate the development of advanced biomarkers, thereapeutic proteins, stem cell (iPS, ES, and MSC) characterization, and also probiotics methodologies; all leading to improved diagnostics, therapeutic treatments, and health enhancement.GlycoStation™ is a Gold Standard in Glycan Profiling

GlycoStation™ Evanescent-field Fluorescence Excitation Glycan Profiler

GlycoStation™ Reader 1200 is an ultra-high speed and highly sensitive optical microarray scanner utilizing the principle of evanescent-field fluorescence excitation to scan an entire microarray slide in only two minutes.

A successor model named “GlycoStation™ Reader 2200” was released in the end of June, 2016.

The scan time is to be greatly improved from 1min40sec to only 10sec at 1sec exposure time condition (Sept., 2017)

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GlycoLite™ 2200, Evanescent-field Fluorescence Excitation Glycan Profiler

GlycoLite™ 2200 is a low cost version of GlycoStation™ Reader 1200 based on the same principle of evanescent-field fluorescence excitation to scan LecChip Ver.1.0.

This product was released in Apr, 2017. The appearance and specifications are subject to change.

Comparative chart between GlycoStation™ Reader 2200 and GlycoLite™ 2200 is shown below.

GlycoStation™ Reader 2200GlycoLite™ 2200
Scanning methodStep & Repeat, sCMOSStep & Repeat, sCMOS
Exposure time133msec – 10sec133msec – 10sec
EMCCD gain none none
Excitation methodEvanescent-field ExcitationEvanescent-field Excitation
Liquid phase detectionpossiblepossible
Scanning area13x65mm Standard, 24x65mm at the Maximum13x65mm
Resolution6.5 µm6.5 µm
Scanning time10sec at the minimum Exposure time, 80sec at the maximum exposure time2min35sec at 1sec Exposure time, 9min20sec at the maximum exposure time
Light sourceMetal halide lampLED
Fluorescence wavelengthCy3, Cy5, FITC (up to 6)Cy3 monochromatic
Digital resolution16bits16bits
Analysis software (Option)GlycoStation ToolsProGlycoStation ToolsPro
Data storageHDD, Exportable through LAN or USB HDDHDD, Exportable through LAN or USB Memory

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